An MPS device for a contractile force model.
Donor cells are split and expanded to prepare enough stock cultures to prepare myobundles. After 4-5 days of growth and expansion, the human skeletal muscle cells are mixed with non-growth factor reduced Matrigel™, thrombin, fibronectin and human growth media. The cell-containing mixture is placed into a mold that helps form the initial muscle bundles. The myobundles are kept in culture for 5-7 days and then the media is changed to differentiation media. The myobundles are kept in the differentiation medium for another 5-7 days. After differentiation the myobundles are tested for their responsiveness to electrical stimulation by developing a contraction.
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