A reproducible 3D neural constructs that incorporates vascular and microglial components derived by culturing precursor cells from the H1 human ES cell line on synthetic hydrogels under defined conditions. Components include di
A reproducible iPSC-derived human 3D brain microphysiological system (BMPS), comprised of differentiated mature neurons and glial cells (astrocytes and oligodendrocytes) that reproduce neuronal-glial interactions and connectivity. BMPS mature over eight weeks and show the critical elements of neuronal function: synaptogenesis and neuron-to-neuron (e.g., spontaneous electric field potentials) and neuronal-glial interactions (e.g., myelination), which mimic the microenvironment of the central nervous system, rarely seen in vitro before.
Spheroids were assayed in 24-well plate.
A reproducible iPSC-derived human 3D brain microphysiological system, comprised of differentiated mature neurons and glial cells (astrocytes and oligodendrocytes) that reproduce neuronal-glial interactions and connectivity. BMPS mature over eight weeks and show the critical elements of neuronal function: synaptogenesis and neuron-to-neuron (e.g., spontaneous electric field potentials) and neuronal-glial interactions (e.g., myelination), which mimic the microenvironment of the central nervous system, rarely seen in vitro before.
The OrganoPlate® 2-lane is an advanced microfluidic tissue culture device that contains 96 independent microfluidic chips. Each chip supports an ExtraCellular Matrix (ECM) channel and a perfused medium lane, with no physical barrier in between. A single chip is connected to four wells of the OrganoPlate®: a gel inlet, two medium reservoirs and an observation window. Any number of chips can be used in an experiment. For central nerve system culture without astrocytes no induced perfusion in the system is required.
This is a 3 cell model with human astroglial cells (ATCC CRL-8621) and human brain vascular pericytes (ScienCell #1200) cultured on the basolateral surface of a collagen pre-coated 12mm transwell and human brain microvascular endothelial cells (ScienCell #1000) on the apical surface. Standard Corning® 12mm PET transwells (catalog no. 3462) coated with rat tail collagen I by the user prior to use. Inserts with 3.0µm Pore Polyester (PET) Membrane, 12mm trans wells in 12 well plates.
This is a 3 cell model with human astroglial cells (ATCC CRL-8621) and human brain vascular pericytes (ScienCell #1200) cultured on the basolateral surface of a collagen pre-coated 6.5mm transwell and human brain microvascular endothelial cells (ScienCell #1000) on the apical surface. Corning® BioCoat® Collagen I Inserts with 3.0µm Pore Polyester (PET) Membrane pre-coated with Collagen Type I extracellular matrix, 6.5mm trans wells in 24 well plates.
This is a 3 cell model with human astroglial cells (ATCC CRL-8621) and human brain vascular pericytes (ScienCell #1200) cultured together on the same side of a collagen pre-coated 24mm transwell and human brain microvascular endothelial cells (ScienCell #1000) on the opposite surface of the membrane. Corning® BioCoat® Collagen I Inserts with 3.0µm Pore Polyester (PET) Membrane were purchased pre-coated with Collagen Type I extracellular matrix, 24mm trans wells in 6 well plates.
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