The WAT MPS mimics the physiological environment of adipose tissue with three main elements: a media channel, circular cell chambers, and a microporous membrane in between.
Analogous to the in vivo blood circulation, media travels through the media channel as a vasculature like microcirculation between multiple WAT chambers and constantly transports fresh nutrients and other soluble factors (e.g. drug compounds, cytokines) to and metabolic waste and secreted factors away from the tissue.
The media channel and WAT chambers are connected via small micropores (diameter 3 μm) that act as a perfusion barrier. The perfusion barrier mimics the in vivo endothelial barrier by allowing nutrients, drugs, and other media compounds to diffuse to the tissue while protecting the cells from shear stresses.
The circular geometry of the WAT chambers (diameter 600 μm, height 50 μm) creates a homogeneous supply with nutrients for the entire WAT tissue and enables the direct exchange of soluble factors with the media for each individual cell, which is important as in vivo each adipocyte is attached to at least one capillary.
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