EpiDerm (MatTek Corporation, MA, USA) is a reconstructed model of human tissue composed of neonatal foreskin-derived keratinocytes. The keratinocytes are cultured on specially prepared permeable cell culture inserts (Millicell CM, Millipore Corp., Bedford, MA, USA) and achieve advanced levels of differentiation characteristic of the mature epidermis. They are organized in basal, spinous, granular and cornified layers with a high concentration of keratohyalin granules and desmosomes.
EpiDerm Full Thickness (MatTek Corporation, MA, USA; EpiDermFT) is a reconstructed model of human tissue composed of normal, human epidermal keratinocytes (NHEK) and normal, human dermal fibroblasts (NHFB) cultured to form a multilayered model of the human dermis and epidermis. The keratinocytes are cultured on specially prepared permeable cell culture inserts (Millicell CM, Millipore Corp., Bedford, MA, USA) and achieve advanced levels of differentiation characteristic of the mature epidermis. EpiDermFT consists of organized keratin 5 expressing basal cells, involucrin and keratin 10 expressing spinous and granular layers, and cornified epidermal layers analogous to those found in vivo. The dermal compartment is composed of a collagen matrix containing viable normal human dermal fibroblasts (NHDF). The epidermal and dermal layers are mitotically and metabolically active and exhibit in vivo-like morphological and growth characteristics which are uniform and highly reproducible. A well-developed basement membrane is present at the dermal/epidermal junction.
The model consists of iPSC-derived keratinocytes form a multilayered epidermis and cornifiedlayer at the surface of the epidermis on a dermis consisting of iPSC-derived fibroblasts, similar to those from human keratinocytes and fibroblasts.
Individual skin constructors are placed into the wells of a 48-well plate.
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