The CN Bio LiverChip is a 3D liver tissue model which is continuously perfused for long-term culture. A scaffold allows formation of 3D tissue structures from primary human hepatocytes, through which culture medium is circulated repeatedly via an onboard pneumatic microfluidic pumping system.
The CN Bio LiverChip is a 3D liver tissue model which is continuously perfused for long-term culture. A scaffold allows formation of 3D tissue structures from primary human hepatocytes, through which culture medium is circulated repeatedly via an onboard pneumatic microfluidic pumping system.
The Liver Acinus MicroPhysiology System (LAMPS) is a new generation of the liver model developed from SQL-SAL 1.5. The model includes 4-Liver cell types model and the Space of Disse
This model is the Nortis Device equivalent to the 96 MCF7 Metastatic Breast Cancer Co-Culture Model. It contains the 4-cell types from the LAMPS model with the addition of the varioius MCF7 mutant cells
In order to verify growth patterns in the LAMPS microfludic model with the addition of MCF7 breast cancer mutant cells a static plate co-culture model consisting of the 4 cell types of the liver and the addition of the MCF7 mutant cells was created.
Remarkable advances in three-dimensional (3D) cell cultures and organ-on-a-chip technologies have opened the door to recapitulate complex aspects of human physiology, pathology, and drug responses in vitro. The challenges regarding oxygen delivery, throughput, assay multiplexing, and experimental complexity are addressed to ensure that perfused 3D cell culture organ-on-a-chip models become a routine research tool adopted by academic and industrial stakeholders. To move the field forward, we present a throughput-scalable organ-on-a-chip insert system that requires a single tube to operate 48 statistically independent 3D cell culture organ models. Then, we introduce in-well perfusion to circumvent the loss of cell signaling and drug metabolites in otherwise one-way flow of perfusate. Further, to augment the relevancy of 3D cell culture models in vitro, we tackle the problem of oxygen transport by blood using, for the first time, a breathable hemoglobin analog to improve delivery of respiratory gases to cells, because in vivo approximately 98% of oxygen delivery to cells takes place via reversible binding to hemoglobin. Next, we show that improved oxygenation shifts cellular metabolic pathways toward oxidative phosphorylation that contributes to the maintenance of differentiated liver phenotypes in vitro. Lastly, we demonstrate that the activity of cytochrome P450 family of drug metabolizing enzymes is increased and prolonged in primary human hepatocytes cultured in 3D compared to two-dimensional (2D) cell culture gold standard with important ramifications for drug metabolism, drug-drug interactions and pharmacokinetic studies in vitro.
iCell hepatocytes are seeded into collagen I gel (4mg/mL) at a density of 15,000 cells/uL in the gel channel. HMEC-1 (20,000 cells/uL) and THP-1 (3,000 cells/uL) are pooled and seeded into the fluidic channel. The device is placed on a rocker for gravity-driven perfusion and media is exchanged every 24h.
This model represents the RED assay conditions and protocols used when testing media serum compound binding for the media used in UCal Berkeley Liver Sinusoid model, as well as derived models from Texas A&M.
This model represents the RED assay conditions and protocols used when testing media serum compound binding for the media used in University of Pittsburgh SQL-SAL 1.5 model, as well as derived models from Texas A&M.
Sequentially layered, self-assembly liver (SQL-SAL). This microfluidic model is a single chamber model with 4 liver cells types and three addition/sample locations associated to it (Influent, Chamber, and Effluent).
Modified SQL-SAL 1.0. This 4-liver cell microfluidic model has a single chamber model and has three addition/sample locations associated to it (Influent, Chamber, and Effluent).
TAMU static, 2D version of the SQL-SAL 1.5 Liver MPS model based on standard 96-well plate. The SQL-SQL 1.5 (3D) is a single chamber model and has three addition/sample locations associated to it (Influent, Chamber, and Effluent).
vLAMPS = Vascularized Human Liver Acinus Microphysiological System.
It is a glass based MPS based on Micronit middle layer but kept under static culture on a 60mm petri dish
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