Enteroid cysts (three-dimensional [3D] structures) are seeded onto permeable membrane supports and grown to confluency to generate monolayers (two-dimensional [2D] structures). The resulting monolayers allow for controllable access to both apical and basolateral surfaces of the intestinal epithelial cells.The monolayer format, as opposed to the classical Matrigel-embedded 3D cyst culture, allows manipulations both at the apical and basolateral cell surfaces in a compartmentalized manner, thereby broadening options for treatment and collection of specimens (i.e. cells and culture media). It also improves reproducibility in evaluation of outcomes by reducing the variation in cell number and the restricted lumen volume inherent in the cysts cultures.
A simple model where primary J2 intestinal cells are seeding into a 96-well plate format.
This model is used for comparison with JHU's transwell enteroid model
This model represents the RED assay conditions and protocol used when testing serum compound binding for the media used in JHU's Intestinal Enteroid model.
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