Bone was grown from adult hMSC capable of osteogenic differentiation within native bone ECM serving as a structural scaffold. An in-vitro model of Ewing’s sarcoma that mimics the key properties of the native tumor and provides the tissue context and physical regulatory signals.
The 3D structures of the bioreactor were modeled using Magics 14 (Materialise, Belgium). The chamber and insert were fabricated using a stereolithography apparatus (EnvisionTec, Germany) employing e-shell 300 as the resin. A multichamber bioreactor was fabricated and fitted into a microfluidic base. An individual bioreactor is composed of a removable insert within a chamber of a well on a 24-well microfluidic plate. The chamber is fixed in place with two O-rings. The osteochondral construct within the insert creates the separation between the upper (chondrogenic) and lower (osteogenic) medium conduits. Opposing gradients of chondrogenic and osteogenic factors and stimulants aid in forming an interface. A single bioreactor is formed by the inset and lid in a well of the plate. Chondrogenic medium (CM) are supplied through upper conduit and osteogenic medium (OM) through the lower conduit.
This model represents the RED assay conditions and protocol used when testing serum compound binding for the media used in Columbia's Bone/Bone-Tumor model.Serum protein concentration is at ??%.
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