A custom-made PDMS mold is placed in each well. The engineered muscle bundles are placed between the beams of a Cerex frame pinned onto the molds. Once, the myobundles are polymerized in the mold for 30 min, they can be cultured in growth media on a rocker (0.33 Hz) at 37°C. On day 4, the media can be switched to differentiation media, and the molds removed leaving the myobundles that are still attached to the frames in the media.
384 well plate platform for high-throughput co-culture and multi-culture applications. This system allows each cell type to be cultured in a well with a media bridge to allow paracrine communication. When media volume is >25μl results in bridged wells and communication between adjacent wells. This version of the well plate is useful for experiments where individual wells cannot be analyzed.
University of Pittsburgh Center for Cellular and Molecular Engineering
The 3D structures of the bioreactor were modeled using Magics 14 (Materialise, Belgium). The chamber and insert were fabricated using a stereolithography apparatus (EnvisionTec, Germany) employing e-shell 300 as the resin. A multichamber bioreactor was fabricated and fitted into a microfluidic base. When the osteochondral construct is inserted, two chambers are formed on either side of the construct (top, chondral; bottom, osseous) that is supplied by different medium streams. These medium conduits are critical to create tissue-specific microenvironments in which chondral and osseous tissues will develop and mature.
The LATTICE platform consists of two elements that are placed in a incubator, a LATTICE culture plate (2UP or 8UP) and a compatible base station, in the 2UP plate up to 2 organ models can be cultured in interconnected culture wells while up to eight organ models can be cultured in the 8UP version. The base stations contains the necessary components to engage and interact with the plate and drive the microfluidic flow and is controlled by proprietary software on a PC. The 2UP base station has one valve mechanism while the 8UP base station has six, as well as several sensors making it compatible with automatic handling.
384-Well Clear Flat Bottom Microplates TC-Treated White Polystyrene
3707
Falcon (Corning)
White 384-well polystyrene microplate has clear flat-bottom square shaped wells with a well volume of 112µL and a recommended working volume of 20 to 80µL
384 well plate platform for high-throughput co-culture and multi-culture applications. This system allows each cell type to be cultured in a well with a media bridge to allow paracrine communication. When media volume is <12.5μl, cells are cultured as a discrete culture. Adding media to >25μl results in bridged wells and communication between adjacent wells.
This glass microelectrode array (MEA) contains a single well with an 8x8 array of electrodes. Surrounding the electrode cluster is a PDMS ring that aids with cell seeding.
The device was produced with two outer housing layers of 0.25” thick transparent poly (methyl methacrylate) (McMaster-Carr, Elmhurst, IL, USA) and two 0.5 mm thick gaskets of CultureWell™ silicone (poly (dimethyl siloxane) PDMS) sheet material (Grace Bio-labs, CWS-S-0.5, Bend, OR, USA) to define the flow path and define positions of the chips.
The versatile microfluidic design contains five chambers for the expansion to additional tissues.
A minimal organoid structure of the heart composed of three components: a central cell chamber, two adjacent media channels, and an array of connecting micro-channels.
• 10μm thick transparent polyester membrane
• Treated for optimal cell attachment
• Packaged 12 inserts in a 12 well plate, 4 plates per case
• Excellent visibility under phase contrast microscopy
• Sterilized by gamma radiation
Transwell cell culture inserts are convenient, easy-to-use permeable support devices for the study of both anchorage-dependent and anchorage-independent cell lines They feature a 10 mm, thin, microscopically transparent polyester membrane that is tissue culture treated for optimal cell attachment and growth.
Eppendorf consumables are the result of over 50 years constant improvement and development. The portfolio of tube products is no exception. Flex-Tube®, with its easy-close design provides ergonomic and reliable one-handed operation. The well-known Eppendorf Tubes 5.0 ml represents the optimal option for dealing with samples volumes that are medium in size. Safe-Lock Tubes are hinged at the lid to provide outstanding protection against unintentional opening during storage and incubation. Protein LoBind tubes are designed specifically for use in proteomics and other areas of protein research. The amount of protein recovered from Protein LoBind tubes is significant for downstream analyses, and the enzymes remain active. Source: https://www.sigmaaldrich.com/labware/labware-products.html?TablePage=17195237
Tested to withstand centrifugation of 12,000 RCF (polypropylene) or 1,800 RCF (polystyrene) in a fully supported rotor with room temperature water. Corning™ Falcon 15mL Conical Centrifuge Tubes are ideal for cell centrifugation; pelleting; separation by density gradients.
The FMi-OOC is composed of four concentric circular cell/collagen chambers designed to mimic the thickness and cell density of the FMi in vivo. Each layer is connected by arrays of microchannels filled with type IV collagen to recreate the basement membrane of the amniochorion.
The photonic biosensor is packaged into a single channel microfluidics stack resting on a temperature-controlled staged where pressure-drive microfluidic flow is provided by an external pump through peristaltic tubing. The sensor is fabricated by AIM Photonics Foundry, the PSA is made by 3M and the silicone tubing is made by Cole Parmer.
The iQue® advanced flow cytometry platform utilizes a fixed wide dynamic range allowing for the collection of both the phenotypes and functional analysis of secreted cytokines simultaneously, eliminating the discrepancy of different time points or the need to split samples for subsequent analysis.
A passive pumping microfluidic chip with up to sixty parallel channels molded with PDMS placed on a plastic/ glass substrate for capture and analysis of immune cells from blood using bright field/ fluorescent microscope imaging.
The LATTICE platform consists of two elements that are placed in a incubator, a LATTICE culture plate (2UP or 8UP) and a compatible base station, in the 2UP plate up to 2 organ models can be cultured in interconnected culture wells while up to eight organ models can be cultured in the 8UP version. The base stations contains the necessary components to engage and interact with the plate and drive the microfluidic flow and is controlled by proprietary software on a PC. The 2UP base station has one valve mechanism while the 8UP base station has six, as well as several sensors making it compatible with automatic handling.
The LATTICE platform consists of two elements that are placed in a incubator, a LATTICE culture plate (2UP or 8UP) and a compatible base station, in the 2UP plate up to 2 organ models can be cultured in interconnected culture wells while up to eight organ models can be cultured in the 8UP version. The base stations contains the necessary components to engage and interact with the plate and drive the microfluidic flow and is controlled by proprietary software on a PC. The 2UP base station has one valve mechanism while the 8UP base station has six, as well as several sensors making it compatible with automatic handling.
Three dimensional (3D) scaffolds, embedded in each of the wells, are continually perfused by cell culture medium during experiments.
The MPS-LC12 consumable plates are designed to create optimal conditions for primary human hepatocytes and non-parenchymal cells (NPCs). Human primary liver cells are cultured in 3D microtissues on an engineered scaffold which mimics the architecture of the liver capillary bed under perfusion. The MPS-LC12 plate can also be used to culture other tissue types, precision cut tissue slices or organoids in a perfused 3D format.
A single luminal structure embedded in a hydrogel.
LumeNEXT, lumen structures are created by utilizing a removable PDMS rod placed in a PDMS chamber. The chamber is filled with an unpolymerized collagen solution that is subsequently polymerized. Once the ECM is completely polymerized, the PDMS rod is removed without disrupting the integrity of the surrounding ECM gel, creating a lumen structure that mimics the geometry of the PDMS rod.
The Mattek Epi-Intestinal model is based on human small intestinal cells (ileum) differentiated after being seeded onto permeable supports under air-liquid interface culture conditions. This commercial model has been well characterized and is available in different formats, including a “full thickness” model in which gut cells are grown on top of a smooth muscle epithelial layer, or a “partial thickness” model with just the gut epithelial cells.
Micronit OOC two-organ (Biomimetic and organoid) series coupling
Micronit
1 Micronit 4 chip holder
2 pairs of 3 layer assembly in glass
-Top and bottom layer with silicon casket
-Middle layers with oval and PET membrane
--1 Middle layer oval (7 x14mm) and PET membrane of 0.45um porous 12um thick (for Biomimetic MPS)
--1 Middle layer oval (8 x16mm) and PET membrane of 3um porous 9 um thick (for encapsulation of organoids)
System is perfused through the top and bottom chambers inlets of the upstream MPS (port 8 and 11). Bottom chamber from upstream MPS is collected through the corresponding outlet (port 2). The top chamber of the upstream MPS (port 5) feeds into the inlet of the top chamber (port 8) downstream MPS. Medium flows through the top chamber and loops (port 5 to 2) into the bottom chamber, with medium collected through the bottom chamber outlet (port 11) of the downstream MPS.
The modular hToC was designed by integrating an ultrathin (100nm) dual-scale (50 nm at 20% porosity with dispersed5 μm pores) silicon nitride membrane (μSiM) into an acrylic-based top component which attaches to a bottom channel through pressure-sensitive adhesive. Two ports on either end of the top component connect to the bottom layer which contains a channel with two rigid horizontal acrylic anchors at either end to provide static axial strain to the tenocyte-embedded TeleCol-3hydrogel.
Muscular thin film (MTF) is an engineered tissue construct, serving as a minimalist system for myogenesis and contractile property readout. MTFs are composed of two layers, one is a supporting layer of biomaterials, the other is a layer composed of muscle cells. The biomaterials can be engineered to provide biochemical and topological cues, so the muscles cells can adhere and assemble into aligned tissue, which truthfully recapitulate their native structure in vivo. This bilayer enables the assessment of contractile stress of muscle tissue in vitro. The contractile force generated by the muscle layer causes the entire film to bend. Based on Stoney’s equation, the resulting curvature of the thin film is used to determine the systolic and diastolic stress from the muscle layer (Feinberg, Feigel et al. 2007, Grosberg, Alford et al. 2011, Shim, Grosberg et al. 2012, Sheehy, Grosberg et al. 2017). External electrical field stimulation can be used to control the frequency of tissue contraction, thus enabling the measurement of stress relationship with frequencies, an important electrophysiological feature.
Frame, when combined with a Matrigel mold, that allows for the growth and support of myobundles. The frame is placed into another container (typically a well in a 24-well plate) that submerges the frame in the various media needed for MPS formation and testing.
Each frame can have a maximum of four functioning myobundles.
Dual chamber system separated by porous 0.2µm pore polycarbonate membrane. Flow through each chamber. There is a vascular chamber that is 2.91 microliters and a brain chamber that is 17.5 microliters. The device thickness is between 4.5-5.5. mm and the substrate thickness is between 3.5-4.5 mm.
-96 independent tissue culture chips
-2 adjacent channels per chip
-Direct access to apical lumen of tubular cultures
Each chip contains one in-gel culture channel and one perfusion channel. This enables the culture of perfused tubules adjacent to an extracellular matrix (ECM) of choice without artificial membranes. With direct access to the apical lumen of tubules, the platform enables perfusion and addition of cells, compounds, and stimuli.
https://www.mimetas.com/en/organoplate-2-lane-96/
Microfluidic chip fabricated from PDMS and bonded to glass. Platinum electrodes were embedded along the media channel and extended 3.2 mm outside the PDMS for attachment to connectors and a pulse generator during electrical stimulation. The PDMS chip featured an inner channel 3.2 mm wide aligned with tapered surface-tension pins set 0.3 mm apart and two PDMS posts of 1 mm diameter spaced 5 mm apart (center to center)
Microfluidic chip fabricated from PDMS and bonded to glass. The PDMS chip featured an inner channel 3.2 mm wide aligned with tapered surface-tension pins set 0.3 mm apart and two PDMS posts of 1 mm diameter spaced 5 mm apart (center to center)
400 um x 200 um x 5 cm (width x depth x length) channel in a PDMS block that is irreversibly bonded to a glass slide. The inlet and outlet have a hole punched out for tubing using a 0.508 mm diameter titanium nitride hole punch. In the middle of the channel, another hole was made using a 400 um diameter punch. This central hole was used to add / remove islets. This hole is covered with PCR tape during experiments.
A 2-layer PDMS chip with a top and bottom chamber separated by a sectioned transwell membrane (0.4uM pore size, polycarbonate). Each chamber is independently fed through an inlet and outlet that allows for gravity flow through the chip. After seeding, the media from the top chamber is removed, bringing cells on the top of the membrane to ALI (Air liquid interface).
The platform is custom-fitted into a standard 96-well plate format. The design consists of two polydimethylsiloxane (PDMS) layers assembled to a commercial 96-well plate (FLUOTRAC™ 600, Greiner Bio-One) with the bottom of specific wells removed to align with the platform. The 2 mm thick middle layer consists of 12 microfluidic device units (denoted as the PDMS device layer) and the bottom layer is a thin transparent polymer membrane (HT-6240, Rogers Corp).
Since liquid evaporation at the corner and edge wells is faster than the inner wells of 96-well plates, only 12 microfluidic device units (U1–U12) are usually arrayed within the inner well area to ensure optimal culture condition. For a single unit, 6 horizontal wells (W1–W6) are utilized. The tissue unit consists of 3 tissue chambers (T1–T3) positioned within the footprint of a single well, with one gel loading inlet (L1) and outlet (L2) located at two additional wells
The MicroBubble (MB) cavity array technology is used as a high-throughput, modular platform. MBs are micron-scale spherical cavities molded in polydimethylsiloxane (PDMS) using gas expansion molding technique. Each spherical MB well has a 100 μm diameter circular opening in the upper third of the well and ~8 nL volume.
The multilayer WAT-chip consists of two patterned polydimethylsiloxane (PDMS, Sylgard 184) slabs sandwiching a polyethylene terephthalate (PET) membrane (rP = 3 μm; ρP = 8 × 105 pores per cm2). The upper PDMS layer features the WAT chamber (green) and the lower one the media channel (red).Multiple cell chambers can be connected in series or in parallel to create different circulation architecture
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