"Sample location" is not applicable to a data point.
Effluent-Brain
Effluent from the brain side of BBB/NVU model. Generally for 3D chips with fluid movement. Also used in case of 2D chips to be specifically compared with 3D chips.
Effluent-Hepatic
Effluent flowing from an MPS model that has more than one chamber, and flow is occurring through the Hepatic chamber (e.g., the hepatic chamber of the vLAMPS model).
Effluent-Apical-Pooled
For some 2D well experiments, the volume of media was insufficient to test, so media from the wells was pooled. These experiments are used for comparison to 3D effluent, so the measurements have been repeated for each well in the group that was pooled prior to measurement.
Basolateral
The basolateral membrane of a polarized cell is the surface of the plasma membrane that forms its basal and lateral surfaces. It faces outwards, towards the interstitium, and away from the lumen.
Effluent
The outlflow from a model with only a single outlet location
Cell-Lysate
A fluid containing the contents of lysed cells
Media-mBrain
The cell culture media that surrounds a brain spheroid / mini-brain / mBrain construct
Effluent-Media
Out-flowing or removed media from an MPS model, well in a plate, etc. Generally used when there is only one sample location.
Stock-Apical
An input (stock) solution that feeds the apical side of an membrane. Example: A solution is made by mixing in a known mass of compound. A sample from the mixture (the stock) is analyzed to determine the actual concentration of the compound in the mixture.
Chamber
The location of the cellular model in a single chamber device. Used when adding or removing directly to the chamber, or for in situ imaging of cells in the model.
Apical
Apical surface of a membrane. Used when adding or removing directly to the apical side of the membrane, or for in situ imaging of cells in the model on the apical side of the membrane.
Effluent-Basal
Effluent from the basal side of a membrane, generally for 3D chips with fluid movement. Also used in case of 2D chips to be specifically compared with 3D chips.
RED-buffer/RED-plasma
Sample location used for reporting the relative amount of compound in the buffer as compared to the plasma side of a RED well plate. Used for Rapid Equilibrium Dialysis (RED) Device Single-Use Plate with Inserts.
RED-buffer
The buffer side of the RED well plate. Used for Rapid Equilibrium Dialysis (RED) Device Single-Use Plate with Inserts.
RED-plasma
The plasma side of a RED well plate. Used for Rapid Equilibrium Dialysis (RED) Device Single-Use Plate with Inserts.
Effluent-Apical
Effluent from the apical side of a membrane, generally for 3D chips with fluid movement. Also used in case of 2D chips to be specifically compared with 3D chips.
Basal
Basal surface of a membrane. Used when adding or removing directly to the basal side of the membrane, or for in situ imaging of cells in the model on the basal side of the membrane.
Membrane
Used when adding or removing directly to the the membrane or when measure the response of the membrane when referring to a side is not appropriate. For example, when measuring transepithelial electrical resistance (TEER) of the membrane.
Cross-Section
Used when, for example, a cross-section sample is collected from an organ model. For example, if a slice of bone is removed from an MPS Model and imaged.
Reservoir-Apical
A pooled remaining solution that feed an apical compartment of an MPS Model. Example: Media of known compound concentration is prepared (stock solution) and loaded into reservoirs feeding multiple MPS Models. After a number of days, what remains in the reservoirs of the multiple chips is pooled together and the concentration of the compound in the pooled mixture is determined. This method of pooling the remaining solution can be employed to provide sufficient volume of media for testing.
Media-Channel
A channel used solely to flow media through a device; there are no cells present in the channel.
This type of location is typically used in devices made by the Healy Lab at UC Berkeley
WAT-Chamber
A circular cell chamber containing white adipose tissue used in the Healy Lab White Adipose MPS model.
Osseous
The bone side (bottom) of an osteochondral junction (OCJ). Use when only one device. If media is flowing through devices in series, the osseous compartments should be numbered sequentially.
Influent
In-flowing solution for a single chamber device with flow.
Effluent-Vascular
Effluent from the vascular side of a model with more than one chamber (e.g., BBB/NVU model). Generally for 3D chips with fluid movement. Also used in case of 2D chips to be specifically compared with 3D chips.
Influent-Hepatic
Influent flowing into an MPS model that has more than one chamber, and flow is occurring through the Hepatic chamber (e.g., the hepatic chamber of the vLAMPS model).
Well
An individual well in a microplate. This is the location were cells are seeded, or where images are taken. This is the equivalent of the Chamber location of the comparative 3D model.
Effluent-Osseous
Media exiting from the bone side (bottom) of an osteochondral junction (OCJ) or from a multiple in series.
Effluent-Chondral
Media exiting from the chondral side (top) of an osteochondral junction (OCJ) or from multiple in series.
Influent-Vascular
Influent flowing into an MPS model that has more than one chamber, and flow is occurring through the Vascular chamber (e.g., the vascular chamber of the vLAMPS model).
Tubule 1 (RPTEC)
RPTEC tubule in Nortis dual-channel chip
Vascular
Chamber of an MPS model with more than one chamber, that functions as a Vascular chamber (e.g., the vascular chamber of the vLAMPS model). Typically used when cells are added to the chamber and/or when images are taken of the vascular chamber.
Hepatic
Chamber of an MPS model with more than one chamber, that functions as a Hepatic chamber (e.g., the hepatic chamber of the vLAMPS model). Typically used when cells are added to the chamber and/or when images are taken of the hepatic chamber.
Media
Media in an MPS model, well in a plate, etc. Used mainly to indicate compound treatment location. When used in a 2D system, this is the equivalent of the Influent and Effluent locations of the comparative 3D model. In a 2D, well based system, media are changed periodically and removed from the well for sampling.
Brain
A chamber of an MPS Model that is modeling the brain.
Chondral
The chondral side (top) of an osteochondral junction (OCJ). Use when only one device. If media is flowing through devices in series, the chondral compartments should be numbered sequentially.
Chondral 1
The first in a series of chondral side (top) of an osteochondral junction (OCJ) when media is flowing through devices in series.
Chondral 2
The second in a series of chondral side (top) of an osteochondral junction (OCJ) when media is flowing through devices in series.
Chondral 3
The third in a series of chondral side (top) of an osteochondral junction (OCJ) when media is flowing through devices in series.
Chondral 4
The fourth in a series of chondral side (top) of an osteochondral junction (OCJ) when media is flowing through devices in series.
Zone 2
The second Zone of the vLAMPS model.
Zone 3
The first Zone of the vLAMPS model. It receives the least oxygenated media.
Interfacial
Surface forming a boundary between adjacent regions (e.g., between chondral and osseous of joint). Use when only one device. If media is flowing through devices in series, the interfacial compartments should be numbered sequentially.
Osseous 1
The first in a series of osseous side (bottom) of an osteochondral junction (OCJ) when media is flowing through devices in series.
Osseous 2
The second in a series of osseous side (bottom) of an osteochondral junction (OCJ) when media is flowing through devices in series.
Osseous 3
The third in a series of osseous side (bottom) of an osteochondral junction (OCJ) when media is flowing through devices in series.
Osseous 4
The fourth in a series of osseous side (bottom) of an osteochondral junction (OCJ) when media is flowing through devices in series.
Influent-Media-Channel
In-flowing solution into the separate Media Channel chamber of the UC Berkeley Healy Lab Heart and Liver MPS models.
Influent-Osseous
Media entering the bone side (bottom) of an osteochondral junction (OCJ) or the inlet to multiple in series.
Influent-Chondral
Media entering the chondral side (top) of an osteochondral junction (OCJ) or the inlet to multiple in series.
Interfacial 1
The first interfacial region in a series of osteochondral junction (OCJ) devices when media is flowing through devices in series.
Interfacial 2
The second interfacial region in a series of osteochondral junction (OCJ) devices when media is flowing through devices in series.
Interfacial 4
The fourth interfacial region in a series of osteochondral junction (OCJ) devices when media is flowing through devices in series.
Interfacial 3
The third interfacial region in a series of osteochondral junction (OCJ) devices when media is flowing through devices in series.
Effluent-Media-Channel
Out-flowing solution from the separate Media Channel chamber of the UC Berkeley Healy Lab Heart and Liver MPS models.
Stock
An stock (input) solution that feeds a one-chamber model of one or more chips/wells.
For example, a mass-spec measurement would be used on media from this location to verify the input concentration of a compound.
Epidermal
The air-facing side of the Columbia University Skin MPS
Tissue
A measurement that comes from a tissue layer in a device
Frame
A supporting structure within a device.
Example: Adding cells to a molding frame for structured growth
Influent-Brain
Inflowing media to the brain side of the Vanderbilt Blood-Brain Barrier model
Sagittal
Imaging a tissue or construct from the sagittal plane
Zone 1
The first Zone of the vLAMPS model. It receives the most oxygenated media.
MEA
Value measured from the integrated MEA (Multi-Electrode Array)
Cells
A measurement that comes from a cells in a device
BoaC Chamber 1
First chamber of Hesperos body-on-a-chip device
BoaC Chamber 4
Fourth chamber of Hesperos body-on-a-chip device
BoaC Chamber 5
Fifth chamber of Hesperos body-on-a-chip device
BoaC Media Port A
Inlet or outlet for media next to Chamber 1 on the Hesperos body-on-a-chip device
BoaC Media Port B
Media inlet or outlet port located next to Chambers 4 and 5 on the Hesperos body-on-a-chip device
BoaC Chamber 2
Second chamber of Hesperos body-on-a-chip device
BoaC Chamber 3
Third chamber of Hesperos body-on-a-chip device
Effluent-Renal
RPTEC channel effluent
Tubule 2 (HUVEC)
Second tubule in Nortis dual-channel chip -- seeded with HUVECs
Media Inlet 1
First media inlet in multi-channel chip
Media Inlet 2
Second media inlet in multi-channel chip
Bioreactor
The central chamber of a the dual flow bioreactor.
Osteochondral-Top
Bone-like tissue, osteochondral-bone in bioreactor
Osteochondral-Bottom
Cartilage-like tissue, Osteochondral-cartilage in bioreactor
Chondral-Top
Cartilage-like tissue in bioreactor
Monophasic chondral-Top
Monophasic chondral tissue
Osteochondral-both
Osteochondral both top and bottom
Glomerular Channel
Channel containing podocytes and glomerular endothelial cells.
Vascular Channel
Channel that remains either empty, or contains endothelial cells
ECM Lane (Mimetas 3-lane organoplate)
The central ECM layer created by adding gel to the phaseguide
SeedEZ scaffold
Lena Biosciences inert, glass microfiber scaffold used with PerfusionPal plates
Empty Channel
A Channel not seeded with cells
Gut Channel
microfluidic channel seeded with gut epithelial cells
Top Channel
Top Channel
Bottom Channel
Bottom Channel
Mid-Channel/Top Channel
Ratio of middle channel to top channel for the Mimetas 3-lane OrganoPlate
ECM Lane (Mimetas 2-lane organoplate)
in gel lane
Effluent - Basolateral
Effluent collected from the basolateral chamber of a model.
perfusion lane
the lane that perfuse media
Input port
Small inlet to allow blood/reagents to be introduced into the device.
Output port
Large outlet to allow for driving passive pumping and removal of blood/reagents from the device.
Channel
Allows for adherence of immune cells and functional assays by brightfield/ fluorescent microscopy.
Microbubble
Single location within a larger, single PDMS array chip.
microJoint-Cartilage
The cartilage component in the microJoint
microJoint-Bone
The bone component in the microJoint
microJoint-fat
The fat component in the microJoint.
microJoint-Synovium
The synovium component in the microJoint
Air channel
Air control channel
Cell type A well
When Media volume <12.5μl, cell type A well behave as a discrete culture. Adding media to >25μl results in cell type A and B bridged wells and cells are cultured as a co-culture.
Cell Type B well
When media volume <12.5μl, cell type B well behaves as a discrete culture. Adding media to >25μl results in bridged wells between Cell type B and A wells and cells are cultured as a co-culture.
microJoint-Synovial fluid
The medium shared by cartilage, synovium and fat tissues
Well Surface
Cell location
DEC Chamber
The DEC chamber is filled with maternal decidual cells in Feto-Maternal Interface
CTC Chamber
The CTC chamber is filled with fetal chorion-trophoblast cells in Feto-Maternal Interface
AMC Chamber
The AMC chamber is filled with fetal amnion mesenchymal cells in the Fetal-Maternal Interface
AEC Chamber
The AEC chamber is filled with fetal amnion mesenchymal cells in the Fetal-Maternal Interface
Top Well of Top Component
Top Well of Top Component in hToC
Top Chamber
Top Chamber of a device
Bottom Chamber
Bottom Chamber of a device
Intravenous
compound or drug administered into a vein or veins
Serum
Blood serum from an in-vivo animal or human model
Urine
Excreted urine from an in-vivo human or animal model
Intraperitoneal
The injection of a substance into the peritoneum (body cavity). It is more often applied to animals than to humans
Testes
Measurement of or extraction from the testes of an in-vivo model
Lumen
In a LumeNEXT device, tubular structure embedded within a collagen matrix
LumeNEXT matrix
In a LumeNEXT device, 3D matrix made with collagen.
Perfusion
Perfusion of a in-situ organ
Drinking Water
In-Vivo animal water intake
Injection
In-vivo injection of a substance
Tissue Chip
PDMS chip
Above membrane
The location above the Transwell membrane surface (location inside of the Transwell insert)
Below membrane
The location below the Transwell membrane surface (location outside of the Transwell insert)
Distal Stimulation
Nerve stimulated at the distal tip of neurite outgrowth, approximately 6-8mm from the recording site in the spheroid region.
Middle Stimulation
The culture is stimulated in the mid-range of neurite outgrowth, ~4-6mm from the recording site in the spheroid region.
Proximal Stimulation
The culture is stimulated in the proximal range of neurite outgrowth, ~2-4mm from the recording site in the spheroid region.
Spheroid Seeding Site
Cells are seeded at one extreme end of the growth-permissive gel allowing only unidirectional growth.
Gavage
Orogastric gavage
Longitudinal Section
Used when a longitudinal section sample is collected from an organ model.
Ventricle Scaffolds
PCL/Gelatin Nanofibrous sheets
Flow Pump
Set the flow rate of peristaltic pump (Masterflex) with a multi-channel pump head (Cole-Parmer) to create continuous flow through the four TEBVs in each chip.
Cell Culture Insert
12 mm, hydrophilic PTFE, 0.4 µm
Provides air liquid interface
Robust holder for four (4) Micronit OOC double chamber
Hepatic chamber
Top chamber of the Micronit double chamber OOC platform.
Pocket section of the middle layer above the PET membrane
Vascular chamber
Bottom chamber of the Micronit double chamber OOC platform.
Section of the middle layer below the PET membrane
Top chamber inlet
Influent flow toward the top chamber of the two-chamber Micronit OOC platform
Bottom chamber inlet
Influent flow toward the bottom chamber of the two-chamber Micronit OOC platform
Top chamber outlet
Effluent flow from the top chamber of the two-chamber Micronit OOC platform
Bottom chamber outlet
Effluent flow from the bottom chamber of the two-chamber Micronit OOC platform
Left A
circuit location-chip location
Right A
circuit location-chip location
Left B
circuit location-chip location
Right B
circuit location-chip location
Left C
circuit location-chip location
Right C
circuit location-chip location
Left D
circuit location-chip location
Right D
circuit location-chip location
Air-Liquid Interface
ALI
Both sides
Added to both apical and basal sides
6-well plate
6-well tissue culture plate
Large Port
Large prot attached to the main chamber
Main Chamber
Central chamber filled with collagen
Top and Bottom compartments
Top compartment (above the membrane, inside of the insert) and bottom compartment (below the membrane, bottom of the well) of a permeable support culture model (i.e. Transwell)
Global
Setting applied to complete system over total time range.
24 WELL ULA PLATE
24 WELL ULA PLATE
Filter
Filter in transwell model
Combined
A combination of all location of a model
Printed Fiber infused gel substrates
Fiber-infused gel ink print 2D anisotropic substrate
Gel inlet
OrganoPlate 2 gel inlet
Perfusion channel inlet
OrganoPlate 2-lane perfusion channel inlet
Flow Cell
The flow cell in a flow cytometer
on top of HCL-LECM
LECM is prepared with 1N HCl followed by sonication, then vortex in hepatocytes platting media.
on top of NaOH-LECM
LECM prepared with 0.1N NaOH with 10X HBSS and hepatocyte platting media, all vortex at once
Above cells
LECM or hydrogel can be placed on top of different cell types
Temporal Neocortex - Left
Human brain structure
Temporal Neocortex - Right
Human brain structure
Forebrain white matter - Left
Human brain structure
Forebrain white matter - Right
Human brain structure
Hippocampus - Left
Human brain structure
Hippocampus - Right
Human brain structure
Parietal neocortex - Left
Human brain structure
Parietal neocortex - Right
Human brain structure
ParVivo SC3 Chip Cell Injection Port
Cell seeding site on the ParVivo SC3 chip.
ParVivo SC3 Source Media Reservoir.
ParVivo SC3 Source Media Reservoir.
Hepatic chamber inlet
Introduction point: allocation of cells, molecules, or particles into the media feeding the hepatic (top) chamber
Vascular chamber inlet
Introduction point: allocation of cells, molecules, or particles into the media feeding the vascular (bottom) chamber of the vLAMPS
Hepatic chamber outlet
Sampling/connection point: efflux collection or connection from the hepatic (top) chamber of the vLAMPS.
Sampling/connection point: collection or connection of cells or particles coming from the hepatic (top) chamber of the vLAMPS
Vascular chamber outlet
Sampling point: efflux collection from the vascular (bottom) chamber of the vLAMPS.
Sampling: collection of cells or particles coming from the vascular (bottom) chamber of the vLAMPS
Alginate encapsulation
Islets are removed from the medium and suspended in 1.1 w/v% low viscosity alginate (Sigma). The islet-alginate mixture placed on top of the membrane and is polymerized with a solution of 100 mM CaCl2 (Sigma) with 10 mM HEPES forming spherical capsules.
Static Media
Media that has not undergone flow
On top of Collagen type 1
Neutralized rat tail Collagen type 1 at a concentration of 50ug/ml, and left to incubate for 30 minutes at 37 degrees Celsius.
Hippocampus
Hippocampus
ParVivo™ SC3 ECM Chamber
The collagen/ECM chamber of the ParVivo™ SC3 ECM Chip
Gen2 Beta Version XYZ chip effluent collection reservoir
This is where effluent is collected for analysis (e.g. LDH or ELISA assays...)
Waste/collection reservoir
effluent collection location
ParVivo SC3 Collection Media Reservoir.
The downstream effluent collection reservoir that media samples are collected from.
Above extracellular matrix
Cells or targets of interest are allocated on top of a biomaterial with the purpose to replicate the extracellular matrix.
Effluent-Total
summation of analyte from vascular and hepatic chambers in vLAMPS model
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